뇌신경세포에서 토양미생물 발효추출액인 석정의 카드윰 독성에 대한 방어효과

Protective Effects of Seok-Jeong on the Toxicity of Cadmium in Neuronal Cells

Seok-jeong (51) is a solution of various metal ions and numerous other organic substances produced through extraction and fermentation of herbs and soil using geo- microbes, and it has been shown to improve symptoms of senile dementia. In this study, we investigated the protective effects of 51 against neurotoxicity of cadmium in HT22 hippocampal neuron cell line. SJ significantly protected from the cadmium- induced decreased cell viability measured by MTT assay (p<0.01). The protective effects of 51 against cadmium toxicity were confirmed through observing morphological changes using inverted microscope. Additional1y; 51 significantly repressed the formation of lipid peroxidation induced by high concentration of cadmium, and likewise, significantly repressed the reduction of glutathione by cadmium in HT22 cells. Vitamin C at the concentration found in 51 did not show any protective effect against cadmium toxicity in HT22 cells, indicating that vitamin C may not have a major role in the protective mechanism of SJ. Taken togethers these results suggest that 51 may be a valuable agent for the protection of cadmium toxicity on the neuronal cells, and that the mechanism of the action of 51 may be due to reduced lipid peroxidation and increased glutathione level.