Protoberberine 알칼로이드가 PCl2 세포중의 L-DOPA 유도 세포독성 작용에 미치는 영향

Effects of Protoberberine Alkaloids on L-DOPA-Induced Cytotoxicity in PC12 Cells

Previously, Protoberberine alkaloids such as berberine and palmatine have been found to lower dopamine content in PCl2 cells (Shin et al., 2000). In this study, the effects of berberine and palmatine on L-DOPA-induced increase in dopamine level and cytotoxicity in PCl2 cells were investigated. Treatment of PCl2 with L-DOPA at concentration ranges of 20~50 μM increased dopamine content and the increase in dopamine levels by L-DOPA was inhibited by 10-40 μM berberine and 10~80 μM palmatine, which the concentration ranges did not show a cytotoxicit7. However, berberine and palmatine at concentrations higher than 50 μM and 100 μM caused a cytotoxicity respectively, In addition, berberine (10~20 μM) and palmatine (10~50 μ M) at non-cytotoxic concentration ranges ageavated L-DOPA-induced cytotoxicity in PCl2 cells (L-DOPA concentration ranges,20~50 μM). The L-DOPA-induced cytotoxicity was also siglificantly potentiated by berberine (50 μM) and palmatine (100 μM) with cytotoxic ranges. These data demonstrate that berberine and palmatine inhibit L-DOPA- induced increase in dopamine content and stimulate L-DOPA-induced neurotoxicity Therefore, the portsibility that the long-term L-DOPA treated patients with berberine and palmatine could be checked the adverse symptoms.