Mungbean Trypsin Inhibitor(MBTI) 및 MBTI-polymer 포합체의 약물학적 특성

Pharmacological Effects of Mungbean Trypsin Inhibitor (MBTI) and MBTI-polymer Conjugate

Mungbean trypsin inhibitor (MBTI) was isolated and purified from Mung bean which has been used as.a galenic and traditional food. MBTI and poly(ethylene glycol) were conjugated by using water soluble carbodilmide. We evaluated the therapeutic value of the MBTI and MBTI-PEG conjugate using animal models, sublethal septic shock model in guinea pig caused by pseudomonal elastase, shock model in rat caused by lipopolysaccharide, and the vascular permeability test by using pseudomonal elastase. In two shock model in guinea pig and in rat, hypotesion shock was inhibited by pretreatment of MBTI. And also the vascular permeability caused by pseudomonal elastase reduced by pretreatment of MBTI. Also, therapeutic value of the MBTI-PEG conjugate was evaluated by using the sublethal septic shock model caused by pseudomonal elastase. The MBTI-PEG conjugate was more effective than native MBTI against pseudomonal elastase induced septic shock in guinea pig model.