한국 성인 분변 시료에서 분리한 비피더스균의 유전자 다양성 분석

Analysis of Genetic Diversity of Bifidobacterium spp. Isolated from Korean AdultsFecal Samples

Twelve strains of Bifidobacterium spp. Were isolated from the feces of healthy Korean 20~30 years. The identification of genera from isolates were performed by the microscopic observation and fructose-6-phosphate phosphoketolase (F6PPK) activity, which is the key enzyme to distinguish the Bifidobacterium spp. From other anaerobic bacteria. To determine the antibacterial resistance patterns, minimum inhibitory concentration (MIC) of several antibiotics (including antituberculosis agents) was determined. Five of the isolates showed the high degree of resistance to vancomycin. To investigate the genetic diversity between isolates and type strain of Bifidobacterium spp. From KCTC, we performed the RAPD-fingerprinting. Using a total set of four primers, it is possible to distinguish the isolates and Bifidobacterium spp. From KCTC. Thus, Bifidobacterium strains isolated from our samples may be a new species or strains of Bifidobacterium genera, and have the potential as a probiotics.