지구자의 Glutathione S-transferase 활성과 알코올 분해효과

Activity of Glutathione S-transferase and Effect of Alcohol Decomposition on the Fruit of Hovenia dulcis Thunb

For the determination of glutathione S-transferase activity, a new method was established by using HPLC system. Moreover, amount of enzyme for a optimum reaction was determined by a comparative study with a variety concentration of enzyme. Using a established method, activity of glutathione S-transferase that is alcohol metabolizing enzyme was investigated on the fruit of Hovenia dulcis Thunb. As the result of experiment, EtOH and H2O extracts of the fruit of Hovenia dulcis Thunb showed visible a synergistic effect of glutathione S-transferase activiy on a continuous experiment, EtOH and H2O extracts of the fruit of Hovenia dulcis Thunb showed alcohol decomposition activity on the in vivo test using rat. These results suggest that the fruit of Hovenia dulcis Thunb may be useful in the prevention of hangover.