HPLC에 의한 가시오가피 및 오가피 중 Hyperin의 정량

Determination of Hyperin in Acanthopanax senticosus and A. sessiliflorus by HPLC

A new high performance liquid chromatographic method was applied for the determination of hyperin in Acanthopanax senticosus and A. Sessiliflorus. The stationary phase used was μ-Bondapak C18 reverse- phase column and a mobile phase program was a gradient of acetonitrile and distilled water at a flow rate of 1.0 ml/min. Hyperin was detected at 210 nm, and the analysis was successfully carried out within 20 min. Hyperin was detected in the one year- grown and two years-grown stem of A. senticosus (0.47 and 0.13 mg/g, respectively) and A. sessiliflorus (0.14 and 0.03 mg/g, respectively). Hyperin was detected in the main and branch root of A. sessiliflorus (0.30 and 0.09 mg/g, respectively). But there is no detection of hyperin in the main and branch root of A. senticosus.