배의 메탄올 추출물이 마우스의 비장세포 증식능과 Cytokine 생성능에 미치는 영향

Effects of Pear(Pyrus pyrifolia) Methanol Extracts on the Proliferation and the Cytokines Production of Mouse Splenocytes

This study was performed to investigate the potential of pear (Pyrus pyrifolia) as a immune-modulating functional food by assay of splenocytes proliferation and induction of cytokines (IFN-γ, IL-4) in vitro. When mouse splenocytes were exposed to various concentration (0.16, 0.31, 0.63, 1.25, 2.50 ㎎/㎖) of pear methanol extracts (P-M) without mitogens, splenocytes proliferation (SP) was significantly increased. Also, SP to mitogens, concanavalin A (Con A) and lipopolysaccharide (LPS) were significantly increased by P-M when compared with controls. When splenocytes were cultured with P-M in the presence of Con A, cytokine (IFN-γ, IL-4) levels in culture supernatant were significantly enhanced in a dose-dependent manner except 2.5 ㎎/㎖ when compared with control group. Therefore, our study suggest that the pear has the potential of being an immune-modulating functional food.