우유속 락토페린의 NMDA 수용체를 통한 진통효과

Effect of NMDA Receptor on Analgesic Effect of Bovine Milk-derived Lactoferrin (BLF)

Lactoferrin is a multifunctional protein that is found in milk, neutrophils, and other biological fluids, and its receptors have also been identified in the central nervous system. Recently, it was reported that bovine milk-derived lactoferrin (BLF) produced analgesia via a μ-opioid receptor-mediated response in the spinal cord. However the precise mechanism of this analgesic effect is remains unclear. In Randall-Selitto paw pressure study, each single administration of morphine (10 mg/kg) and BLF (50, 100 and 200 mg/kg) induced analgesia, however, NMDA receptor antagonist MK-801 (0.1, 0.2 and 0.3 mg/kg), inhibited analgesia induced by BLF(100mg/kg). Intracerebroventricular infusion (I.C.V) of N-methyl-D-aspartic acid (NMDA) (0.3 μg/8.0 μl/hr/day), as a NMDA receptor agonist, reversed inhibition of MK-801 (0.3mg/kg) on analgesia induced by BLF (100mg/kg). These results suggest that BLF have analgesic effect, through NMDA receptor activation.