Sasa borealis의 Diastereomeric 성분들의 역상 고속액체크로마토그래프 분석방법

Reverse-Phase HPLC Method for Identification of Diastereomeric Constituents from Sasa borealis

Reiterated normal-phase column chromatography lead to the isolation and purification of six known compounds but for the first time from the whole plant of Sasa borealis (Hack.) Makino (Gramineae); tricin 4'-O-(erythro-β-guaia-cylglyceryl) ether (1), tricin 4'-O-(threo-β-guaiacylglyceryl) ether (2), tricin 4'-O-[erythro-β-guaiacyl-(9"-O-acetyl)-glyceryl] ether (3), tricin 4'-O-[threo-β-guaiacyl-(9"-O-acetyl)-glyceryl] ether (4), (-)-pinoresinol (5), and vanillin (6). The structures of the compounds (1-6) were established based on interpretation of high resolution NMR (COSY, HSQC, HMBC, and NOESY) spectral data. In particular, compounds 1 and 3 were diastereomers of compounds 2 and 4, respectively. These two sets of diastereomers were able to be simultaneously identified and quantified by a gradient reversed-phase HPLC method with UV photodiode array. This sensitive HPLC method is noteworthy as a simultaneous separetion and identification method to test the extract of the family Gramineae which contains these compounds.