한국 성인에서 분리한 유산균의 VISA (Vancomycin-Intermediate Resistant Staphylococcus aureus)와 VRE (Vancomycin Resistant Enterococcus faecium)에 대한 성장 억제

Lactic Acid Bacteria Isolated from Healthy Korean Having Antimicrobial Activity Against VISA and VRE

Visa and VRE are the main causes of surginal infection, urinary tract infections and bacteremia in hospitals. In this study, we selected VISA (Vancolycin Intermediate resistant Staphylococcus aureus) and VRE (Vancomycin Resistant Enterococcus) isolated from the clinical isolates, One of the isolated strains indicated the high resistance to several anti-biotics (Vancomycin, Teicoplanin, Mupirocin, Synercid, Ciprofloxacin, Lincomycin, Cefotaxim, Meropenem). Antimicribial activity of Bifidobacterium spp. against VISA and VRE were measured. About 104cells of VISA or VRE were mixed with 1,5 and 9 ㎖ of Bifidobacterium and the final volume was adjusted to 10㎖ with brain heart infusion (BHI) broth. The cell suspension was incubated for 3, 6, 9,and 24 hr, serially diluted and then plated on BHI agar plate. As numbers of Bifibobacterium were increased viable cell count of VISA and VRE decreased. The strongest antimicrobial activity of the Bifibobacterium was observed after 9hr incubation in any mixture, almost completely inhibiting the growth of VISA and VRE.