강활 에틸아세테이트 분획에 의한 PG 분해 iNOS, PGE2, 활성 억제 및 진통효과

Analgesic Activity and Inhibitory Effect of PG Degradation, iNOS and PGE2 by Ethyl Acetate Fraction of Angelica Koreana Radix

Prostaglasdins biosynthesis and nitric oxide production have been implicated in the process of inflammation In this study, we investigated on the effects of ethyl acetate extract of Angelica Koreana Radix (EAKR) on the activities of prostaglandin E2 (PGE2), proteoglycan (PG) degradation and nitric oxide synthase (NO) in inflammation cytokines-activated rabbit articular chondrocytes. EAKR exhibited inhibitory activities on NO production and PGE2 production as 73.08% and 89.49%, respectively at 20 ㎍/㎖ and inhibited the degradation of PG in a concentration-dependent manner. Zelatin zymog- raphy analysis demonstrated that EAKR significantly inhibited MMR-2, 9 expression in chondrocytes. In vivo, EAKR was shown to have inhibitory effects on acetic acid-induced pain. This study suggests that modulation of PGE2, NO, PG degradation and MMP-2, 9 by EARK may be important in the prenebtion of inflammation and osteroarthritis.