Tributyltin 화합물이 PC12 세포의 Dopamine 생합성 저해작용에 미치는 영향

Inhibitory Effects of Tributyltin Acetate on Dopamine Biosynthesis in PC12 Cells

The effects of tributylin acetate (TBTA), one of the endocrine-disrupting organotin compounds, on dopamin biosynthesis in PC 12 cells were investigated. Treatment of PC12 cells with TBTA at 0.05-0.25 ㎛ significantly decreased the intracellular dopamin contest in a comcentration-dependent manner (IC50value, 0.17 ㎛). Under these conditions, tyrosine hydroxylase (TH) activity and TH mRNA level were also decreased by 0.1 ㎛ TBTA at 24h, and recovered there- after, In addition. treatment with L-DOPA at 20 and 50 ㎛ increased the intracellular dopamin contest in PC12 cells and the increase in dopamine contest by L-DOPA was significantly aboloshed by TBTA at 0.1-0.2 ㎛. These results indicate that TBTA at 0.1~0.2 ㎛ causes the decrease in the basal dopamin contest and abolishes the increase in dopamin content in L-dopa-treated cells in part by the inhibition of TH gene expressin and activity.