황금 지상부의 항산화 및 알러지 활성 성분

Antioxidant and Antiallergic Activity of Compounds from the Aerial Parts of Scutellaria baicalensis Georgi

Roots of Scutellaria baicalensis have been used for fever remedy, diuresis, antiphlogistic. For the investigation of the active component from aerial parts of Scutellaria baicalensis, MeOH extracts from aerial parts of Scutellaria baicalensiswere suspended with H2O, and partitioned by CHCl3+ In order to investigate the efficacy of antioxidative activity, the activity guided fraction and isolation of physiologically active substance were performed. Its H2O, 30%, 60% MeOH and MeOH frac- tions were examined on antioxidative activity using DPPH method and TBARS assay. It was revealed that 30% and 60% MeOH fractions have significant anti-oxidative activity. its fractions testing type I allergy, compound 48/80 induced systemic anaphylaxis by 71% and 57%, respectively, From 30%, 60% MeOH fraction, five compounds were isolated and elucidated apigrnin 6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (isoschaftside, I), scutellarein 7-O-β-D-glucuronopyranoside (scutellarin, Ⅱ), apigrnin 7-O-β-D-glucuronopyranoside (Ⅲ), isoscutellarein 8-O-β-D-glucuronopyranoside (Ⅳ), kaempferol 3-O-β-D-glucopyranoside (Ⅴ) through their physicochemical data and spectroscopic methods. We measured radical scav- enging activity with DPPH method and anti-lipid peroxidative efficacy on human L이 with TBARS assay. 〔Ⅰ<Ⅳ≒Ⅴ≒Ⅲ<Ⅱ〕showed antioxidant activities in order. Type I allergy, compound 48/80 induced systemic anaphylaxis was applied.〔Ⅴ< Ⅱ<Ⅳ〕inhibited systemic anaphylaxis in order.