사이토카인 생산에 미치는 생지황메탄올추출물의 효과

Effect of Fresh Rehmanniae Radix Methanol Extracts on the Production of Cytokines

We investigated the effect of fresh Rehmanniae radix methanol extracts (RGMeOH) in the in vitro production of cytokines by splenocytes and perigoneal macrophages isolated from C57BL/6 mice. Peritoneal macrophages and sple- nocytes were incubated with various concentrations of RGMeOH in the presence of 10μg/ml of lipopolysaccharide (LPS) or 1μg/ml of concanavalin A (Con A) for cytokine assay. These results showed that RGMeOH remarkably attenuated LPS-increased production of TNF-α but IL-6 by peritoneal macrophages and enhanced LPS-stimulated production of IL-10 in a dose-dependent manner. RGMeOH significantly augmented the LPS-or Con A-stimulated production of IL-2 and IFN-y by splenocytes. These findings suggest that RGMeOH may attenuate inflammatory responses through down-regulation of TNF-α and up-regulation of IL-10, and RGMeOH may up-regulate cell-mediated immune responses through increase in IL-2 and IFN-y production.