흰털오가피 부위별 물추출물의 항산화활성

Antioxidation Activity of Water Extract of Different Parts of Acanthopanax divaricatus var. albeofructus

Acanthopanax species have traditionally been used as a tonic, a sedative as well as in the treatment of rhcumatism, hypertension and diabetes. In the present study, oxidative stress was induced in Vero cells by incubating the cells with glucose and the cell viability was measured by MTT assay. The concentration of glucose which 50% of cell viability was 125 mM (IC50) and the cell viability was increased to 87.6±8.8% by treatment of the extracts of Acanthopanax divar- icatus var. albeofructus. The antioxidative activity of water extract of different parts of the Acanthopanax plant was investigated by DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, xylenol orange assay, TBARS (thiobarbituric acid reactive substances) assay and enzyme (superoxide anion and catalase) assay. Each extract (leaves, root, stem and fruits) of the plant showde free radical and H2O2 scavenging activity. The extract also inhibited lipid peroxidation and recovered enzyme(superoxide anion dismutase and catalase) activity in Vero cells treated with glucose.