하수오 지상부의 활성 성분

Active Compounds from The Aerial Parts of Polygonum multiflorum Thunberg

Polygoni Radix (Polygonaceae) has been used as renal disorder, hematopoietic, menstrual irregularity and anti-aging in Korean folk medicine. Specially aerial parts has been used for insomnia and sedative agent. In order to investigate the efficacy of antioxidant activity on these aerial parts, the bio-activity guided fraction and isolation of physiologically active substance were performed. H2O, 30%, 60%, 100% MeOH were examined antioxidant activity by DPPH method. It was revealed that 30%, 60% MeOH fractions have significantly antioxidant activity. From 60% MeOH fraction, six known flavonoid glycosides (quercetin, quercetin 3-O-β-D-galactopyranosyl-4'-O-glucopyranoside-3'-methylether, quercetin 3-O-α-L-rhamnopyranoside, quercetin 3-O-α-L-rhamnophyrano side-4'-methylether, quercetin 3-O-β-D-galactopyranoside, quercetin 3-O-β-D-glucopyranoside) and 3,4-dihydrocinnamic acid were isolated. To investigated the antioxidant activities of each compounds, we measured radical scavening activity with DPPH method and anti-lipid peroxidative efficacy on low density lipo-protein(LDL) with TBARS assay and anti-acetylcholinestrase activity with Ellman's Test. Four compounds (Ⅱ,Ⅳ,Ⅵ,Ⅷ) of quercetin glycosides showed significant activity.