Farnesol의 HL-60 세포에 대한 세포독성과 활성산소 및 황산화효소 활성 변화

Reactive Oxygen Species and Antioxidant Enzyme Activities in Accordance

Farnesol in fruits, vegetables, herbs and leaves acts as bioactive component with pervention of cancer and phychologica malaise. We investigated the cytotoxic effects of farnesol on human leukemic cell, HL-60 cells, by MTT assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Farnesol (0.1~50 μg/ml) exhibited cytotoxicities against HL-60 cells, the generation of reactive oxygen species such as O2 and H2O2 were found to be considerably increased. The most prominent augmentations of O2 and H2O2, superoide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities of HL-60 cells treated with farnesol were measured. SOD and GPx activities were found to be remarkably elevated by addition of farnesol showing the best results of 273% and 167% of controls, respectively. All data suggest that farnesol may have played as an apoptosis inducer in HL-60 cells via production of reactive oxygen species (ROS) and HL-60 cells may have failed to overcome the damage of ROS on account of still deficient ROS scavengers including SOD and GPx.