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아미노산 Phenylthiohydantoin 유도체를 키랄 고정상으로 한 GC에 의한 아미노산 광학 이성질체의 상호분리

Separation of Amino Acid Enantiomers by Gas Chromatogaphy on Phenylthiohydantoin Derivatives as Chiral Stationary Phases

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The resolution of five amino acids derivatives by gas chromatography on phenylthiohydantoin derivatives as chiral stastionary phase was investigated. (S)-5-isopropyl, isobutyl, (sec)-butyl and benzoyl derivatives of N3-phenyl-2-thiohydantoin were synthesized from L-valine, L-leucine, L-isoleucine and L-phenylalanine, respectively. The phases were employed at several column temperatures (140oC-210oC). The separation factors were compared with each stationary phase with varying R groups. The possible mechanism of chiral recognition was investigated by NMR technique.

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