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Bacillus licheniformis EMR-1에서의 MLS 유도내성 기전 erm K의 크로닝

MLS Inducible Resistance Mechanism in Bacillus licheniformis EMR-1 Cloning of erm K, a MLS Resistance Determinant

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Inducible MLS resistance gene of Bacillus licheniformis specified by erm K was subcloned in Bacillus subtilis, and the DNA sequence corresponding to its control region was determined. The determinant erm K was in Pvu II=Hind III fragment, which was 1.3 kb. The leader region is capable of forming a complex series of inverted complementary repeat sequences(ICRS) centering on at least six axes of symmetry, some of them mutually exclusive, in a way that resulted ultimately in post-transcriptional unmasking of the ribosome loading site for methylase synthesis.

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