금은화(Lonicerae Flos)의 Ethyl Acetate 분획이 돌연변이원성에 미치는 영향

Effects of Lonicerae Flos'' Ethyl Acetate Fraction on Mutagenicity

Based on the following tests with fractions extracted from organic solvents such as benzene, chloroform, ethyl acetate and methanol, this study is to analyse the antimutagenicity of Lonicerae Flos. When carrying out Ames test with Salmonella typhimurium strain, it seemed that there was stronger antimutagenicity in TA 100 treated by MNNG than did one in TA 98 by NPD, and that there was stronger antimutagenicity through base pair exchange than one through frame shift. In the umu test, each fraction tended to inhibit the activity of beta-galactosidase induced by AF-2. As shown in these above tests, the ethyl acetate fraction was the strongest among four fractions. On the other hand, its component consisted of luteolin, apigenin, chlorogenic acid and five unknown ingredients. Of these unknown ingredients, E, F strongly tended to inhibit the activity of beta-galacosidase. In addition, there was also the decrease in its activity of apigenin, luteolin and chlorogenic acid.