Electroblotting을 이용한 단일클론항체의 NH2-말단 아미노산 배열분석

NH2-terminal Amino acid Sequence Analysis of Monoclonal Antibody by Electroblotting Method

NH2-terminal amino acid sequence analysis of monoclonal antibody is very important to identify gene family and diversities of antigen-antibody recognition. When we used the PVDF (Polyviriylidene difluoride) membrane blotting method, we could easily analyze NH2-terminal sequence of monoclonal-antibody which specifically binds to phosphatidylinositol 4,5-biphosphate. PVDF membrane is an ideal solid-phase support for sequence analysis, especially when used with electroblotting method. This method is superior to continual method and will be applied to the sequence analysis of picomole quantities of proteins by gel electrophoresis.