간접 분광 검출법에 의한 음이온의 분석(I)

Analysis of Anions by Indirect Photometric Detection (I)

An ion chromatographic method based on indirect photometric detection of UV transparent anions was developed. Separation of anion was accomplished on strong anion exchange column (Waters SAX) using UV detector at 254 nm. Among examined UV-active additives (Dns-H, Dns-glu, Dns-ser, Dns-val), Dns-glu showed the highest sensitivity. Studies on the effects of the pH and ionic strength of eluent revealed that the increase of pH and ionic strength of the eluent decreased capacity factor. The best eluent for the separation of acetate, fluoride, chloride, nitrate and bromide was 1X10-4M Dns-glu in 5mM phosphate buffer (pH 6.30). The detection limit of chloride ion was 2.1ng in this condition.