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Radio-HPLC에 의한 [3H]-Benzo(a)pyrene 대사물의 분석

Analysis of [3H]-Benzo(a)pyrene Metabolites by HPLC with Radioactive Flow Detection

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A modified method was investigated for the determination of benzo(a)pyrene metabolites generated by the rat liver microsomes based on the HPLC technique with radioactive flow detection. By adding [3H]-dexamethasone to the B(a)P metabolites mixture metabolized by the microsome, the poor yield of solvent extaction of B(a)P metabolites was compensated.

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