MLS계 항생물질 유도내성 유전자의 크로닝과 유전자의 발현조절 기전 Staphylococcus aureus TR-1균주의 프라스미드 pMB4에 존재하는 MLS 내성 유전자 ermC-4

Cloning of Inducible MLS Antibiotics Resistance Genes and their Expression Control Mechanism ermC-4, a macrolide-lincosamide-streptogramin B resistance determinant on pMB4 from Staphylococcus aureus TR-1

pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0 Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.