병원성 원생동물의 변이와 약물내성: Leishmania major의 클로로퀸 내성

Drug Resistance in Protozoan Parasites : Chloroquine Resistance in Leishmania major

A comparative study was performed on two different strains of Leishmania majorchloroquine sensitive strains (ChlS) and its mutant chloroquine resistant strains (ChlR). ChlR strains were obtained at 5X10-4M chloroquine. Remarkable differences were observed at the initial chloroquine uptake in ChlR and ChlS, ie., the rate of uptake was very reduced in ChlR (Km values were 70nM and 125nM, respectively). Influx and accumulation of chloroquine were also compared between wild type and mutant. An increasing tendency in both influx and accumulation of chloroquine was shown in ChlS, but ChlR demonstrated a rapid release after a little uptake (influx) at the early stage. This result is thought to be basis of their resistance for ChlR strains.