감초가 면역반응에 미치는 영향(I) -50% 메탄올 엑스의 면역조절작용-

Effect of Glycyrrhizae Radix on the Immune Responses(I) -Immuno-regulatory Action of 50% Methanol Extract

These experiments were conducted to investigate the effects of Glycyrrhizae Radix extract(GR) on histamine synthesis, lymphocyte blastogenesis in C57BL/6J mice splenocytes, IL-1 production, Ca2+ uptake by macrophage-like P388D1 cells and plaque forming cell assay against SRBC. Histamine contents, lymphocyte blastogenesis, IL-1 activity, Ca2+ uptake and plaque forming cell were determined by enzyme isotope method, [3H]-thymidine incorporation, C3H/HeJ mouse thymocytes proliferation, the addition of 5mcCi/ml 45Ca2+ to P388D1 cell suspension and assay to sheep red blood cell, respectively. Cytotoxicity, which was expressed as 50% mortality, was occurred by the addition of GR(10-3g/ml). Histamine production in mouse spleen cell culture was significantly increased by 48 hour incubation added 0.25mcg/ml of Con A. Con A-dependent T-lymphocyte proliferation was also enhanced by the addition of 0.25mcg/ml of Con A. GR depressed histamine contents at 10-9-10-4mcg/ml and Con A (0.25 mcg/ml) dependent T-lymphocyte proliferation at 10-5-10-4g/ml. IL-1 activity was significantly decreased by 10-8-10-4g/ml of GR. Ca2+ uptake was not changed by GR, but antibody production markedly increased at 10.0-50.0mg/kg of GR. From the above results, it is suggested that GR have immuno-regulatory action; GR decreased cell-mediated immune response and increased antibody production by B lymphocyte at high doses.