감초가 면역반응에 미치는 영향(II) -Glycyrrhizin 및 Glycyrrhetinic acid의 면역조절작용-

Effect of Glycyrrhizae Radix on the Immune Responses(II)- Immuno-regulatory Action of Glycyrrhizin and Glycyrrhetinic Acid -

These experiments were conducted to investigate the effects of glycyrrhizin(GL) and glycyrrhetinic acid(GA) on histamine synthesis, lymphocyte blastogenesis in C57BL/6J mice splenocytes, IL-1 production, Ca2+ uptake by macrophage-like P388D1 cells and plaque forming cell assay against SRBC. Histamine contents, lymphocyte blastogenesis, IL-1 activity, Ca2+ uptake and plaque forming cell were determined by enzyme isotope method, [3H]-thymidine incorporation, C3H/HeJ mouse thymocytes proliferation, the addition of 5 mcCi/ml 45Ca2+ to P388D1 cell suspension and assay to sheep red blood cell, respectively. Cytotoxicity, which was expressed as 50% mortality, was occurred by the addition of GL(10-3M) and GA(10-4M). Histamine production in mouse spleen cell culture was significantly increased by the addition of 0.25 mcg/ml of Con A, after 48 hour incubation. Con A dependent T-lymphocyte proliferation was also enhanced by the addition of 0.25 mcg/ml of Con A. The effects of GL on histamine contents and T-lymphocyte proliferation were significantly decreased at high dose (10-5M), while IL-1 activity was remarkably suppressed by 10-8 - 10-4M of GL. Ca2+ uptake was not changed, but antibody production was increased by GL(10mg/kg). GA inhibited histamine contents at 10-9 - 10-7M and depressed Con A (0.25mcg/ml) dependent T-lymphocyte proliferation at 10-7 - 10-5M of GA, but increased suboptimal dose (Con A 0.1mcg/ml) at 10-9 - 10-7M of GA. IL-1 activity was suppressed by 10-8 - 10-4M of GA and Ca2+ uptake was enhanced by 10-9 - 10-6 of GA, but antibody production was not changed by GA. From the above results, it is suggested that GL and GA have immuno-regulatory action. GL decreased cell-mediated immune response, and increased humoral immune response at high dose. On the other hand, low dose of GA enhanced cell-mediated immune response, while high doses of GA decreased humoral immune reaction.