N-Cyanoaziridine 유도체의 합성 및 항암효과 평가

Synthesisof 2,3-Alkyl Substituted N-cyanoaziridines and Their in vitro Cytotoxicity

Seven derivatives of 2,3-alkyl substituted N-cyanoaziridine were stereoselectively prepared from alkyl substituted alkenes and cyanamide. NMR spectral property and conformation of N-cyanoaziridine were analysed. Protons(3.34 ppm) at 2,3 position of N-cyanoaziridines are significantly deshielded in case of cyclopentyl fused N-cyanoaziridine. These protons are getting more anisotropically shielded with increasing the fused carbocycle size of N-cyanoaziridine. Therefore chemical shift for these protons are upfield shifted to 2.80 ppm in case of cyclooctyl fused N- cyanoaziridine. Their ED50 values aganist L1210 cell in vitro were evaluated as 0.5-8.0 mcg/ml. Conformation of carbocycle of fused N-cyanoaziridine is more important to their cytotoxicity than the increment of the strain energy of 3-membered ring.