플라보노이드배당체에 의한 Bacteroides JY-6의 beta-글루코시다제 및 alpha-람노시다제의 유도

Induction of beta-Glucosidase and alpha-Rhamnosidase of Bacteroides JY-6 by Flavonoid Glycosides

Optimal medium for growth and glycosidases production of Bacteroides JY-6, an human intestinal bacterium, was general anaerobic medium or tryptic soy broth containing sodium thioglycolate and ascorbic acid. By cocultivation of Staphylococcus R-48, Bacteroides JY-6 could be cultured in LB broth unable to culture JY-6. Heated Staphylococcus R-48 was also the inducer of the production of Bacteroides JY-6 glycosidases. These glycosidases were induced well by natural flavonoid glycosides, such as poncirin, naringin and rutin, but were not by synthetic substrates, p-nitrophenyl beta-D-glucopyranoside and p-nitrophenyl alpha-L-rhanmopyranoside.