혈소판 및 Na+, K+-ATPase, cyclicAMP 포스포디에스테라제에 대한 해양천연물질의 작용

Inhibition of Na+,K+-Atase, cyclicAMP Phosphodisterase and Platelet Activation by Secondary Metabolites form Marine Organisms

The purpose of this investigation was to determine the inhibition of Na+,K+-ATPase, cyclicAMP phophodiesterase and platelet activation by secondary metabolites isolated from marine organisms. The secondary metabolites were isolated and identified as six diterpenoids(1 : astrogorgin, 2 : ophirin, 3 : calicophirin B, 4, 5 and 6 : cladiellin) from the dichloromethane extract of Muricellajsp., four ceramides(1,2,3, and 4) from Acabaria undulata and three antharaquinones(1,2 : crysophanol, and 3 : physcion) from Urechis unicintus. The results demonstrated that diterpenoids(2,3, and 4) showed the inhibition of cyclicAMP phosphodiesterase, and ceramides(1,3, and 4) showed the inhibition of cyclicAMP phosphodiesterase and thrombin(0.1 units/ml)-induced aggregation of washed rabbit platelet, and anthrapuinones((1,2, and 3) showed the inhibition of Na+,K+-ATPase. Among the anthraquionones, 1,2-dimethoxy-3-methyl-8-hydroxy-anthraquinone(1) showed the inhibition of collagen(1.0 mcg/ml)-induced aggregation in a concenration-dependent manner with IC50 value of 42.8 mcM.