학술저널
고속액체크로마토그래피에 의한 벤조일 클로라이드를 유도체화제로 한 혈청 중의 폴리아민의 분리 정량
Determination of Polyamines in Serum Using Benzoyl Chloride as Derivatization Agent by HPLC
- 대한약학회
- 약학회지
- 제41권 제4호 (1997년)
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1997.08414 - 420 (7 pages)
- 12
A rapid and sensitive high performance liquid chromatographic method with UV detection for the analysis of polyamines(putrescine, spermidine, spermine) has been developed. The benzoyl chloride derivatives of putrescine, spermidine and spermine are separated on a mc-Bondapak C18 reverse-phase column with 50% methanol as the mobile phase. This method was applied to the analysis of polyamines in the serum of normal human and uterine cancer patients. The results show that the mean level of polyamines in cancer patients serum is much higher than that in normal human serum.
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