혈광평활근 수축에 대한 타이로신 인산화 억제제의 효과

Effects of Tyrosine Phosphorylation Inhibitors on Vascular Smooth Muscle Contraction

We studied effects of genistein and tyrphostin, inhibitors of tyrosine kinase, on contractions induced by high K+ and norepinephrine in rat aorta. Genistein (10-6~10-4M) and tyrphostin (10-5~10-4M) inhibited high K+ and norepinephrine-induced sustained contractions, respectively in a concentration-dependent manner. High K+ and norepinephrine caused an increase in 45Ca2+ uptake while 10-4M genistein and tyrphostin inhibited the K+ and norepinephrine-increased 45Ca2+ uptake, respectively. These results show that inhibitor of tyrosine kinase blocks the voltage-and receptor-operated Ca2+ channels in rat aorta, respectively.