1,2,4-트리메틸벤젠에 피폭된 노동자의 뇨 중 3,4-디메틸히푸르 산과 크레아티닌의 정량

Determination of 3,4-Dimethylhippuric Acid and Creatinine from the Urine of Workers Exposed to 1, 2,4-Trimethylbenzene

Creatine and 3.4-Dimethylhippuric acid (3,4-DMHA), a glycine conjugate of 1.2,4-trimethylbenzene (1,2,4-TMB) were determined in the urine of workers exposed to 1,2,4-TMB vapor. The best condition for the simultaneous determination of 3.4-DMHA and creatine by high performance liquid chromatography was obtained by reverse phase C18 column (4.6X150mm, 5mcm) as stationary phase and 20% acetonitrile in 20mM phosphate buffer (pH 3.0) containing 4mM sodium octylsulfate(SOS)as mobile phase. The recovery of 3,4-DMHA spiked to blank urine in the range of 1~5mcg/ml was about 96%. The concentration of urinary 3,4-DMHA of workers had a positive correlation with the environmental level of 1,2.4-TMB (r=0.866). The data suggest that urinary 3,4-DMHA concentration is a useful biological index for 1,2.4-TMB exposure.