학술저널
해모필루스 K-12가 생산하는 황산전이효소의 고정화
Immobilization of Arylsulfate Sulfotransferase Obtained from Haemophilus K-12
- 대한약학회
- 약학회지
- 제42권 제1호 (1998년)
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1998.0153 - 58 (6 pages)
- 10
A novel type of sulfotransferase, arylsulfate sulfotransferase (EC 2.8.2.22) purified from Haemophilus K-12, an intestinal bacterium of a mouse, was immobilized onto AH-Sepharose 4B, CH-Sepharose 4B and DEAE-celluose. The enzyme was stabilized for storage more markedly by covalent immobilization onto AH-Spharose 4B or CH-Sepharose 4B and by adsorptive immobilization onto DEAE-cellulose than the free enzyme. The optimal pH and acceptor substrate specificity of immobuized enzyme were similar to those of the free enzyme.
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