사염화탄소로 독성을 유발시킨 일차배양 간세포에 미치는 G009의 효과

Hepatoprotective Effect of G009 on CCl4-induced Hepatotoxicity in Primary Cultured Rat Hepatocytes

G009, a polysaccharide isolated from the mycelia of Ganoderma lucidum IYO09, showed a hepatoprotective activity against CCl4 induced cytotoxicity in primary cultured rat hepatocytes. Incubation of CCl4-intoxicated hepatocytes with G009 significantly reduced the levels of glutamic pyruvic transaminase and sorbitol dehydrogenase released from hepatocytes in the medium. G009 showed antioxidative effect by elevating the activities of glutathione reductase and superoxide dismutase, and the content of glutathione in CCl4-intoxidcated primary cultured rat hepatocytes. Furthermore, G009 significantly elevated glutathione-S-transferase activity in CCl4-intoxicated primary cultured rat hepatocytes. G009 also reduced the production of malondialdehyde, a byproduct of lipid peroxidation. From these results, it could be concluded that G009 exerted hepatoprotective activity against CCl4-induced cytotoxicity through antioxidation.