강활에서 종양괴사인자 생성을 억제하는 활성성분의 분리

Isolation of Inhibitory Components on Tumor Necrosis Factor-alpha Production from Angelica Koreana)

The total ethanol extract of Angelica koreana radix (UmbeUiferae) showed potent inhibitory effect on tumor necrosis factors (TNF-alpha) production from LPS-stimulated RAW264.7 cells in screening studies on 118 Korean medicinal plants. The activity-guided fractionation of the total ethanol extract resulted in the isolation of 4 compounds, including active substances. The chemical structures of the compounds isolated were established by chemical and spectrometric analyses as imperatorin, isoimperatorin, osthol and oxypeucedanin. Among them, osthol, oxypeucedariin and isoimperatorin showed significant inhibitory activities on TNF-alpha production as 54.3+/-2.1%, 33.6+/-1.0% and 17.7+/-3.0%, respectively, at 12.5mcg/ml.