췌장 베타세포에서 인터루킨-1beta로 유도한 인슐린 의존형 당뇨병 실험 모델

Prediabetic In vitro Model in Pancreatic Beta Cells Induced by Interleukin-1beta

To establish prediabetes in vitro/ model concerning the etiology of Insulin Dependent Diabetes Mellitus (IDDM) in cellular level we have designed experimental prediabefic model in pancreatic beta cells. RINm5F, HIT-T15 and isolated rat islets were chosen as pancreatic beta cells. Since interleukin-1beta-induced beta cell cytotoxicity has been implicated in the autoimmune cytotoxicity of IDDM, we used inteleukin-1beta as diabetogenic agent. For establishment of prediabetic in vitro model, the degree of beta cell deterioration was determined by cell proliferation, insulin release and morphological appearance. Cell proliferation, insulin release and morphology were changed dose-dependently in condition that inteleuldn-1beta was exposured to pancreatic beta cells. The concentration and exposure time of interleukin-1beta to set up prediabetic model in beta cell lines and isolated rat islets were 100-1000U/ml, 48hr. And 25-100U/ml, 48hr, respectively.