수치에 의한 황금의 성분변화

Change of Flavonoid Composition in Scutellaria baicalensis by Processing

Scutellaria baicalensis Georgi (Labiatae) has been extensively used in treatment of diarrhea, conjunctivitis, gastritis, enteritis and skin disease. As instructed in old description, the Scutellariae Radix should sometimes be processed before use. To shed light on the changes of chemical compositions by processing as well as processing method-activity relationships, Scutellariae Radix was heated at 160oC-200oC for 30 min. in furnace or soaked with 20% EtOH (SPE) and boy`s urine(SPU), respectively, which are one of processing methods and then heated at 170oC for 30 min. To isolate the chemical components, Scutellariae Radix with/without processing were extracted with EtOH and EtOH exbract was fractionated with ether, ethyl acetate and butanol to give respective fractions. Ether and EtOAc fractions obtained from the processed drug with urine (SPU) were subjected to chromatography to obtain six components, oroxylin A, wogonin, chrysin, baicalein, baicalein 7-0-beta-D-gucopyranoside and beta-si-tosterol 3-0-beta-D-gucopyranoside. All the isolated compounds were identified by means of physico-chemical and spectroscopic methods (IR 1H-NMR, 13C-NMR, Mass). By HPLC determination, the changes of the contents for each isolated components from SPE and SPU samples were observed. It was found that the content of nonglyecosidic flavones such as oroxylin A, wogonin, chrysin and baicalein was increased markedly, whereas the content of baicalin and baicalein 7-O-beta-D-glu-copyranoside was signaficantly decreased in both samples as compared with those of Scutellariae Ra-dix. When the sample was soaked with boy`s urine, the total amount of nonglycosidic flavones was higher than that of processing with 20% EtOH.