천연 나린게닌의 항허피스바이러스작용과 아시클로버 및 비다라빈과의 병용효과

Antiherpetic Activities of Natural Naringenin Alone and in Combinations with Acyclovir and Vidarabine

To search for less toxic antiherpetic agents, the inhibitory effects of natural naringenin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by the plaque reduction assay in vitro. Naringenin inhibited plaque formations of HSV-1 and HSV-2 in a dose dependent manner. It also exhibited more potent antiherpetic activity on HSV-1 with selectivity index (SI) of 19.1 than on HSV-2 with SI of 5.7. The combined antiherpetic effects of narin genin with nucleoside antiherpetic agents, acyclovir and vidarabine, were examined on the multiplication of these two strains of herpesviruses in Vero cells by the combination assay. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combinations of naringenin with acyclovir on HSV-1 and HSV-2 showed more potent synergism with CI values of 0.28-0.81 for 50%, 70%, 90% effective levels than those with vidarabine with CI values of 0.86-3.28.