Carrageenin으로 처치한 흰쥐 발 염증에서 Indomethacin에 의한 유도성 nitric oxide synthase의 발현증가

Potentiation of Inducible Nitric Oxide Expression by Indomethacin in Carageenin-treated Rat Paw Inflammation

Present study was aimed to examine whether indomethacin affected the production of NO in the rat paw exudate by carrageenin. Paw edema and nitrite/nitrate levels in the paw exudate were maximal after 4h and remained elevated up to 10h, whereas indomethacin (10mg/kg. Po) significantly inhibited the carrageenin-induced paw edema and levels of nitrite/nitrate in the paw exudate. However, paw edema and nitrite/nitrate levels were increased thereafter for 10h. Indomethacin also enhanced the expression of iNOS mRNA and protein 4h after carrageenin injection. Indomethacin inhibited the level of PGE2 in the paw exudate in a time-dependent manner. These results suggest the possibility that indomethacin may potentiate expression of iNOS and subsequently increase nitrite/nitrate level in the late phase of carrageenin-induced rat paw inflammation possibly by suppressing cycloxygenase activity.