감마선에 조사된 생쥐에 있어서 방사선방어효과 평가를 위한 생물학적 파라메타

Biogical Parameters for Assessing Radioprotective Effects in gamma-irradiated Mice

This study deals with the biological changes in mice after gama-irradiation. Four weeks old BALB/c mice were irradiated with 6.5Gy of gamma-ray on the fifth day after oral administration of radioprotectants such as ascorbic acid, tocopherol and cysteine. Control group was irradiated with 6.5Gy without pre-administration of radioprotectors. Blood cells and sperm cells were counted and body, testis and spleen were weighed 3 days after irradiation. And also liver antioxidant activity and range of spleen immune cells were measured. Differences in most biological parameters were not clearly distinguished between experimental groups. However, the relative spleen weight, the relative testis weight and the population size of spleen immune cells such as T helper cells, B cells and macrophages measured by means of FACS showed significant difference between irradiated and radioprotectant administered group. It is concluded that the relative spleen weight, the relative testis weight and the population size of spleen immune cells are easy and useful parameters for assessing the effect of radioprotective substances and for quantifying biological damage of radiation, as well.