HPLC-ECD를 이용한 흰쥐의 뇌 중 감마 아미노부티르산 및 글루탐산의 정량

Determination of gamma-Aminobutyric Acid and Glutamic Acid in Rat Brain by High Performance Liquid Chromatography with Electrochemical Detection

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and gamma-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with 0-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.