고삼추출물의 항균효과 (Ⅰ)

Antimicrobial Effect of the Extract of Sophora flavescens Ait (Ⅰ)

In order to develop a antimicrobial agent, dried Sophora flavescens Ait. was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration(MIC) of the extracted substance against microorganisms were also examined. Antimicrobial activity of the initial methanol extract from the sample was the strongest compared to those of other solvent extracts such as H2O, n-hexane, chloroform, ethyl acetate and methanol. The methanol extract had strong growth inhibition activity against gram-postive bacteria (MIC, 25~50mcg/ml) such as B. subtilis and S. aureus. Among gram positive bacteria tested, B. subitlis was the most susceptible to the extracted substance. The antimicrobial activity of the methanol extract from the sample had strong growth inhibition activity against gram-negative bacteria such as P. aeruginosa (MIC, 25mcg/ml).