모세관전기영동장치 및 고속액체 크로마토그래피에 의한 황금의 성분 분석법 비교

Studies on the Comparison of Scutellaria Radix Analyses by CE and HPLC

A simple, accurate and reproducible Capillary electrophoresis (CE) assay has been developed for the determination of baicalin, baicalein, wogonin and chrysin in Scutellaria baicalensis. Successful separation of these compounds has been obtained in 35mM phosphate buffer (pH 7.0) using a untreated fused silica capillary (57cm×75mcm I.d.) at 25o with the electric field of 19kV. Baicalin, baicalein, wogonin and chrysin was separated and detected at 280nm 13min. The detection limits of CE were acceptable compared to HPLC. Reproducibilities of migration time and peak area were 0.66~1.11% (within-run), 2.18~3.38% (between-run) and 3.50~4.55% (within-run), 3.97~4.82% (between-run) at CE. The results indicate that CE could be a promising technique for quality and quantity control analysis of Scutellaria baicalensis as a validation method.