황해쑥(Artemisia argyi)의 H9(ATCC HTB176)세포에 대한 세포독성 및 항산화효소 활성

Cytotoxicity of Artemisia argyi Extract Against H9(ATCC HTB176) Cell and Antioxidant Enzyme Activities

The hot water and methanol extracts of Artemisia argyi showed considerable cytotoxicities against H9(ATCC HTB176) cancer cell with IC50 values of 48.6mcg/ml and 50.9mcg/ml, respectively. These cytotoxicities were found to be dependent on the extract concentrations and culture days. CuZnSOD and MnSOD activities were significantly increased in the cytoplam and mitochondria fractions of cancer cell, and media in the presence of Artemisia argyi. Such enhanced SOD activities were generally in the range of two to threefolds. In contrast to SOD, catalase and glutathione peroxidase activities were not detected at all. These results suggest that Artemisia argyi have generated O2- in the mitochondria and cytoplasm of H9 cancer cell with concurrent induction of CuZnSOD and MnSOD in situ, which dismutate O2- to H2O2. Without coordinated actions of catalase and/or gluathione peroxidase H2O2 is easily converted to very toxic OH and these reactive oxygen species together might have induced nectosis and/or apoptosis of H9 cell.