천연감미료 스테비오사이드와 스테비올의 생체내, 시험관내 유전독성평가

In vitro and In vivo Evaluation of Genotoxicity of Stevioside and Steviol, Natural Sweetner

The standard operation procedure of mouse lymphoma L5178Y tk+/- -3.7.2C gene mutation assay (MOLY) has been regarded as a sensitive In vitro mammalian cell gene mutation assay that is capable of detecting clastogens as well as mutagens. Using MOLY, one of natural sweetner, stevioside (5mg/ml) and its aglycon, steviol (340mcg/ml) were evaluated the mutagenicity. Stevioside and steviol did not induce mutagenicity in MOLY. On the other hand, stevioside (250mg/kg, B.W.) and steviol (200mg/kg, B.W.) were also evaluated their ability to induce mucronuclei in regenerating hepatocytes and bone marrow cells of ddY mice. From these results, stevioside and steviol did not induce any mutagenic effect both MOLY and In vivo micronucleus test.