별불가사리의 항돌연변이 활성

Antimutagenic Activity of Asterina pectinifera

The antimutagenic activities of the total extract and several fractions of starfish, Asterina pectinifera (Asteriidae), were investigated in vitro by SOS chromotest with Escherichia coli PQ37 and Ames test with Salmonella typhimurium TA100. When various fractions was tested, the chloroform and butanol fractions showed low induction factors, which means both fractions increased antigenotoxicity against the base substitution mutagen MNNG. Even though higher antigenotoxic effect of the chloroform fraction, no effective result of Ames test was found in revertant formation of S. typhimurium TA100. The most effective antigenotoxic and antimutagenic fraction was a butanol one: I.e., When 0.5mg/tube of butanol fraction was applied, the induction factor was 0.68. As the concentration of the fraction was increased, an induction factor was significantly reduced to 0.47. The 2.5mg/tube of the fraction potently inhibited the formation of revertants of S. typhimurium TAIOO by about 81%. There was no cytotoxic effect of butanol fraction against S. typhimurium TA100. This result might be useful for further study to search a possible anticancer agent from the starfish, Asterina pectinifera.