Vibrio vulnificus cytolysin의 흰쥐 혈소판 응집 기전

Mechanism of Vibrio vulnificus Cytolysin on Rat Platelet Aggregation

Vibrio vulnificus cytolysin has been incriminated as one of the important virulence determinants in V. vulnificus infection. In the present study, the effects of Vibrio vulnificus cytolysin on platelets were examined. Vibrio vulnificus cytolysin induced platelet aggregation and increased intracellular calcium concentration ([Ca2+]I) of rat platelets. These effects were abolished in Ca2-free buffer (2mM EGTA). Cytolysin also potentiated ADP- and collagen-induced platelet aggregation. Lanthanum (2mM) inhibited cytolysin-induced platelet aggregation. However, another Ca2+ channel blockers, verapamil (20mcM) or mefenamic acid (20mcM) did not block cytolysin-induced platelet aggregation. Osmotic protectants, sucrose (50mM) and raffinose (50mM) suppressed platelet aggregation by 35.9% and 63.4%, respectively. V. vulnificus cytolysin increased membrane conductances of platelet membranes. These results suggest that cytolysin-induced platelet aggregation is mediated via lanthanum sensitive-calcium influx which resulted from the pore formation by V vulnificus cytolysin.