신성 고혈압 흰쥐의 초기단계에서 내피 의존적인 혈관반응의 변화

The Alteration of Endothelium-Dependent Vascular Response at Early Stage of 1K1C Renal Hypertensive Rats

We investigated whether endothelium-derived NO and endothelin-1 might result in the enhanced vasoconstriction induced by administration of norepinephrine (NE) at the early stage of one-kidney, one-clip (1K1C) renal hypertensive rats. We also studied the relationship of renin-angiotensin system (RAS) using rat aorta in this hypothesis. L-NMMA (30mcM) and L-NAME (30mcM) enhanced vasoconstriction induced by NE in thoracic aorta of control rats. However angiotensin converting enzyme (ACE) inhibitor didn't. The aorta of 1K1C rats showed a significantly exaggerated contractile response to NE as compared with control rats. Rubbing the endothelium abolished this difference. Ach and SNP-induced vasorelaxation show no significant difference between 1K1C and control rats. The treatment of phosphoramidon (10mcM) and oral administration of captopril (0.05, w/v%) abolished the exaggerated contractile response to NE at early stage of 1K1C rats. These results suggest that the increase of contractile response at the early phase in 1K1C rat is partially involved in the activation of ACE.