뇌졸중 치료 생약 추출물의 흥분성 신경독성 억제효과

Inhibition of Excitotoxic Neuronal Cell Death By Total Extracts From Oriental Medicines Used For Stroke Treatment

The methanol extracts were prepared from 46 oriental medicines currently used for stroke treatment, and the effects were assessed on the excitotoxic neuronal cell death induced by L- glutamate(Glu) in primary cultured rat cortical neurons. The extracts from Angelicae gigantis Radix, Manitis Squama, Acori graminei Rhizoma, Uncariae RamWus et Uncus, Alpiniae Fructus, Paeoniae Radix, and Cnidii Rhizoma inhibited the Glu-induced neurotoxicity with the IC50 values of 95.2, 218.6, 263.3, 295.1, 297.9, 310.1, and 446.7mcg/ml, respectively. The extracts from Arisaematis Rhizoma, Loranthi Ramulus, Anemarrhenae Rhizoma, Carthami Flos, Clematidis Radix, Bambusae Concretio Silicea, and Angelicae koreanae Radix also exhibited significant inhibition of the toxicity. In contrast, the extracts from Aconiti Tuber, Araliae cordatae Radix, Curcumae Rhizoma, Leonuri Herba, Polygalae Radix, Salviae Radix, and Siegesbeckiae Herba increased the Glu-induced toxicity at the concentrations of 500 and 1000mcg/ml. Rest of the extracts evaluated in the present study showed minor or negligible inhibition. Taken together, the oriental medicines including Angeficae gigantis Radix, Manitis Squama, Acori graminei Rhizoma, Uncariae Rwnulus et Uncus, and Alpiniae Fructus appear to exert pharmacological effects through the inhibition of excitotoxic neuronal cell death. Further studies are in progress to characterize active principles in these extracts.